The goal of the project is to investigate the local intestinal immune status and its aging role in people over 95 years old living in Kazakhstan
In healthy aging, centenarians are a successful model, exhibiting relatively low rates of various diseases and improved long-term health compared to older people. In the aging process, variation in the gut microbiome plays a key role, which is facilitated by the diet, genetics and
lifestyle of older people. In this regard, the study of the gut microbiome / mycobiome of long-lived people may lead to an understanding of how, with increasing longevity, the gut microbiota adapts to changes and contributes to the maintenance of metabolic and immunological homeostasis of the body, as well as supports quality aging and increases the survival of the body.
To achieve the main goal of the project, we have identified the following tasks:
1) Recruiting people over 95 years old and respondents of a different age group to the control group
The respondents will be invited to participate in the study and sign informed consent of persons over 95 years old and persons of different age categories. A comprehensive clinical and laboratory examination will be carried out. And the collection of biomaterials (blood, rectal swabs) was carried out and the nutritional assessment of the elderly and centenarians was carried out using the Food Frequency Questionnaire (FFQ). An electronic metadata base of all research participants will be created.
2) Study of the features of local immunity of centenarians (over 95 years old) in comparison in other age groups
Immune status studies will be carried out using multiplex analysis for the following indicators: sCD40L, EGF, Eotaxin / CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL- 1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AA , PDGF-AB / BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF, IgA, IgG1-G4, IgM.
3) To study the composite bacterial and fungal composition of the gut microbiome of the elderly and centenarians
DNA extraction will be performed from all samples to analyze the gut microbiome and mycobiome. Zymo Research swabs will be used to collect samples and DNA extraction will be performed using the ZymoBiomics DNA Microprep Kit (Zymo Research), further sequencing will be performed on the Illumina MiSeq platform. Less functional script of taxonomic units (LotuS) will be used to analyze the obtained data.
4) Comparative analysis of the gene expression of intestinal senescence (proteins p16) among the population over 95 years old and other age categories
Gene expression of intestinal senescence will be investigated for aging markers p16 in study participants over 95 years old and in other age categories.
5) Conduct a comparative and correlation analysis of nutritional indicators with the gut microbiome and local immune status in different age groups
Correlation analysis will be performed using the R version 3.6.2 software. All indicators obtained as a result of completing the first three tasks will be used for the analysis. There will be a publication in a peer-reviewed scientific publication, Q1 / Q2, or having a percentile in the
Scopus database of at least 50 and 1 article in a peer-reviewed foreign and (or) domestic publication with a non-zero impact factor (recommended by the CCSON Ministry of education of the Republic of Kazakhstan).
Expected results
As a result of the implementation of the project objectives, the following results will be achieved:
1) A comprehensive clinical and laboratory examination of people over 95 years old and people of different age categories will be carried out, with the taking and interpretation of the relevant tests (blood, rectal swabs)
2) An assessment of the common and local immune status of study participants over 95 years old and a control group of study participants of different age categories will be carried out
3) The compositional microbial composition of the intestines of persons over 95 years old and the control group of different age categories will be studied.
4) A genetic biomarker of intestinal senescence (protein p16) will be determined in study participants over 95 and in other age groups.
5) Correlation analysis of indicators of common and local immune status and microbiome /mycobiome will be carried out
In healthy aging, centenarians are a successful model, exhibiting relatively low rates of various diseases and improved long-term health compared to older people. In the aging process, variation in the gut microbiome plays a key role, which is facilitated by the diet, genetics and
lifestyle of older people. In this regard, the study of the gut microbiome / mycobiome of long-lived people may lead to an understanding of how, with increasing longevity, the gut microbiota adapts to changes and contributes to the maintenance of metabolic and immunological homeostasis of the body, as well as supports quality aging and increases the survival of the body.
To achieve the main goal of the project, we have identified the following tasks:
1) Recruiting people over 95 years old and respondents of a different age group to the control group
The respondents will be invited to participate in the study and sign informed consent of persons over 95 years old and persons of different age categories. A comprehensive clinical and laboratory examination will be carried out. And the collection of biomaterials (blood, rectal swabs) was carried out and the nutritional assessment of the elderly and centenarians was carried out using the Food Frequency Questionnaire (FFQ). An electronic metadata base of all research participants will be created.
2) Study of the features of local immunity of centenarians (over 95 years old) in comparison in other age groups
Immune status studies will be carried out using multiplex analysis for the following indicators: sCD40L, EGF, Eotaxin / CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL- 1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AA , PDGF-AB / BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF, IgA, IgG1-G4, IgM.
3) To study the composite bacterial and fungal composition of the gut microbiome of the elderly and centenarians
DNA extraction will be performed from all samples to analyze the gut microbiome and mycobiome. Zymo Research swabs will be used to collect samples and DNA extraction will be performed using the ZymoBiomics DNA Microprep Kit (Zymo Research), further sequencing will be performed on the Illumina MiSeq platform. Less functional script of taxonomic units (LotuS) will be used to analyze the obtained data.
4) Comparative analysis of the gene expression of intestinal senescence (proteins p16) among the population over 95 years old and other age categories
Gene expression of intestinal senescence will be investigated for aging markers p16 in study participants over 95 years old and in other age categories.
5) Conduct a comparative and correlation analysis of nutritional indicators with the gut microbiome and local immune status in different age groups
Correlation analysis will be performed using the R version 3.6.2 software. All indicators obtained as a result of completing the first three tasks will be used for the analysis. There will be a publication in a peer-reviewed scientific publication, Q1 / Q2, or having a percentile in the
Scopus database of at least 50 and 1 article in a peer-reviewed foreign and (or) domestic publication with a non-zero impact factor (recommended by the CCSON Ministry of education of the Republic of Kazakhstan).
Expected results
As a result of the implementation of the project objectives, the following results will be achieved:
1) A comprehensive clinical and laboratory examination of people over 95 years old and people of different age categories will be carried out, with the taking and interpretation of the relevant tests (blood, rectal swabs)
2) An assessment of the common and local immune status of study participants over 95 years old and a control group of study participants of different age categories will be carried out
3) The compositional microbial composition of the intestines of persons over 95 years old and the control group of different age categories will be studied.
4) A genetic biomarker of intestinal senescence (protein p16) will be determined in study participants over 95 and in other age groups.
5) Correlation analysis of indicators of common and local immune status and microbiome /mycobiome will be carried out