Development of a methodology for the restoration of the mucosal layer of the intestine after antibiotic therapy

Objective of the project:
To establish the pathogenetic features of the destabilization of the microbiocenosis system of the gastrointestinal tract, changes in the state of the mucosal layer during therapy with antibacterial drugs and develop methodologies for restoring the microbiocenosis system and the mucosal layer of the intestine after antibiotic therapy.

Project tasks:

1. Study of the structure and dynamics of changes in the intestinal microbiocenosis system under the influence of antibacterial drugs.
The implementation of this task involves the recruitment of patients with infectious / non-infectious pathology, a comprehensive clinical and laboratory examination, collection of biomaterial, transportation of biomaterial to a research laboratory, analysis of local immunity for 32 indicators (intestines), analysis of intestinal microflora. The analysis of the biomaterial will be carried out after the start of antibiotic therapy on days 1, 3 and 5.
2. Study of the fundamental foundations of changes in the structure of the mucosal layer under the action of antibacterial drugs.
Within the framework of this task, experimental studies will be carried out on laboratory animals with the measurement of immunological, microbiological, histological, etc. indicators.
3. Study of the dynamics of spontaneous recovery of the microbiocenosis system and the structure of the intestinal mucosal layer after the completion of antibiotic therapy with and without various therapeutic effects.
As part of this task, the collection and analysis of biomaterial will be carried out after the completion of antibiotic therapy on days 1, 3, 5, 30 and before the restoration of the intestinal microflora.
4. Development of a Protocol for the restoration of the microbiocenosis system and the mucosal layer of the intestine after antibiotic therapy.
As part of this task, methodologies will be developed and guidelines for restoring the intestinal mucin layer after antibiotic therapy will be published.