Project goal
The main aim of the project is pre-clinical testing of the new composites for fracture bone regeneration based on the modifications of the tricalcium phosphate (TCP) ceramic porous granules with Strontium (Sr) and Copper (Cu).
Project tasks:
Abstract: Bioceramics and, in particular, calcium-phosphate-based materials are widely recognized for their excellent biocompatibility, osteoconductivity, and their ability to induce biomineralization. In the present project, tricalcium phosphate (TCP) ceramic porous granules modified with Strontium (Sr) and Copper (Cu) will be developed. Our choice of the metals is dictated by the fact that Sr has been reported to possess osteogenic and bone regeneration properties while Cu is capable of increasing the antibacterial and angiogenic potential of the TCP granules. Sr is present in the mammalian bones’ mineral phase. It is hypothesized that Sr plays a role in the early bone formation and induction of the positive effects on osteogenesis, osteoclastogenesis, and angiogenesis. Furthermore, Sr has positive therapeutic effects on osteoporosis and osteoarthritis. Sr has been also shown to increase osteoblast proliferation, differentiation, and mineral formation, and to slow down the osteoclastic resorption of the bone. Sr-doped hydroxyapatite has a positive effect on osseointegration and bone regeneration, according to our experience and literature reports. The use of Cu in the biomedical field is desirable because it is an essential cofactor of several enzymes and possesses proangiogenic and antibacterial properties. In this regard, in this project, we propose to assess the efficiency of the deposition of TCP granules modified with Sr and Cu in vitro and in vivo. We suggest that implantation of the TCP ceramic porous granules modified with Sr and Cu in the zone of bone fracture will be an effective method for stimulating reparative osteogenesis and may improve the outcomes of pathological bone fractures.
The main aim of the project is pre-clinical testing of the new composites for fracture bone regeneration based on the modifications of the tricalcium phosphate (TCP) ceramic porous granules with Strontium (Sr) and Copper (Cu).
Project tasks:
- In vitro assessments of the effects of TCP ceramic porous granules modified with Sr and Cu on osteogenic differentiation and migration properties of bone marrow-derived mesenchymal stem cells (BM-MSCs) and evaluation of its anti-osteoclastic activity
- In vitro evaluation of the angiogenic potential of TCP ceramic porous granules in the HUVEC model and assessment of its biocompatibility and antibacterial properties
- In vivo evaluation of the therapeutic potential of the TCP ceramic porous granules modified with Sr and Cu in a rat model of the ulna fracture
Abstract: Bioceramics and, in particular, calcium-phosphate-based materials are widely recognized for their excellent biocompatibility, osteoconductivity, and their ability to induce biomineralization. In the present project, tricalcium phosphate (TCP) ceramic porous granules modified with Strontium (Sr) and Copper (Cu) will be developed. Our choice of the metals is dictated by the fact that Sr has been reported to possess osteogenic and bone regeneration properties while Cu is capable of increasing the antibacterial and angiogenic potential of the TCP granules. Sr is present in the mammalian bones’ mineral phase. It is hypothesized that Sr plays a role in the early bone formation and induction of the positive effects on osteogenesis, osteoclastogenesis, and angiogenesis. Furthermore, Sr has positive therapeutic effects on osteoporosis and osteoarthritis. Sr has been also shown to increase osteoblast proliferation, differentiation, and mineral formation, and to slow down the osteoclastic resorption of the bone. Sr-doped hydroxyapatite has a positive effect on osseointegration and bone regeneration, according to our experience and literature reports. The use of Cu in the biomedical field is desirable because it is an essential cofactor of several enzymes and possesses proangiogenic and antibacterial properties. In this regard, in this project, we propose to assess the efficiency of the deposition of TCP granules modified with Sr and Cu in vitro and in vivo. We suggest that implantation of the TCP ceramic porous granules modified with Sr and Cu in the zone of bone fracture will be an effective method for stimulating reparative osteogenesis and may improve the outcomes of pathological bone fractures.