Chromosome-scale assembly of Kazakh individuals using modern genomics approaches
Project goal: Creation of a chromosomal-level assembly of whole genomes of individuals of the Kazakh population using data from long and short sequencing reads, as well as data from bionano optical genomic maps and Hi-C chromosomal conformation
Project description: The idea of the project is to use genomic data obtained from modern high-throughput genomic platforms and bioinformatics methods to create the best chromosomal-level assembly of whole genomes of individuals of the Kazakh population and make it available for exploitation as a reference population-oriented genome in various biomedical investigations.
Project facilitators: PI: Ulykbek Kairov;
Project participants: Asset Daniyarov, Askhat Molkenov, Kuanysh Sarkytbayev, Anel Ordabayeva.
Realisation period: 2024-2026
Expected results: - The quality of long and short sequencing reads will be assessed for subsequent de novo assembly of whole genomes;
- De novo assembly of whole genomes of Kazakhstani individuals will be carried out based on data from long sequencing reads;
- Integration of data from short sequencing reads will be carried out to polish and improve the assembly of whole genomes;
- Hybrid integration of long-read assemblies with bionano genomic maps will be carried out to improve draft genomic assemblies;
- Hi-C chromosomal conformation data will be mapped on the reference genome and hybrid genomic assemblies;
- The quality of draft and hybrid genome assemblies will be assessed;
- Comparative analysis of the resulting draft and hybrid assemblies will be carried out with genomic data from other populations and individuals;
- The results of the best assemblies will be deposited in open access, making them available to use in comparative, population, and biomedical analysis by researchers in the field of genomics and bioinformatics;
Project description: The idea of the project is to use genomic data obtained from modern high-throughput genomic platforms and bioinformatics methods to create the best chromosomal-level assembly of whole genomes of individuals of the Kazakh population and make it available for exploitation as a reference population-oriented genome in various biomedical investigations.
Project facilitators: PI: Ulykbek Kairov;
Project participants: Asset Daniyarov, Askhat Molkenov, Kuanysh Sarkytbayev, Anel Ordabayeva.
Realisation period: 2024-2026
Expected results: - The quality of long and short sequencing reads will be assessed for subsequent de novo assembly of whole genomes;
- De novo assembly of whole genomes of Kazakhstani individuals will be carried out based on data from long sequencing reads;
- Integration of data from short sequencing reads will be carried out to polish and improve the assembly of whole genomes;
- Hybrid integration of long-read assemblies with bionano genomic maps will be carried out to improve draft genomic assemblies;
- Hi-C chromosomal conformation data will be mapped on the reference genome and hybrid genomic assemblies;
- The quality of draft and hybrid genome assemblies will be assessed;
- Comparative analysis of the resulting draft and hybrid assemblies will be carried out with genomic data from other populations and individuals;
- The results of the best assemblies will be deposited in open access, making them available to use in comparative, population, and biomedical analysis by researchers in the field of genomics and bioinformatics;