Head of the Laboratory of Biophotonics Imaging, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana, Kazakhstan, h-индекс: 23

Ivan Vorobyev



1979 – Ph. D. in Cytology, Moscow State University, USSR

1975 – Research Specialist, Moscow State University, USSR


Prof. Ivan Vorobyev earned his Ph.D. in Dept. of Cell Biology and Histology, Moscow State University. Since that he held research positions at A.N. Belozersky Institute for Physico-Chemical Biology, Moscow State University and for many years continued research on the centrosome structure and dynamics of microtubules. He described in detail centriole fine structure (1980) and relationship between centriole cycle and cell cycle (1982). Together with D. Zorov he discovered in 1983 mitochondria fragmentation. He earned Doctorate of Sciences degree in Cytology in 1989. In 1997 described free microtubules as ubiquitous part of cytoskeleton in animal cells. He supervised more than 15 graduate students and was advisor for 10 Ph.D. thesis. In 1988-2015 he was PI on 14 grants from Russian Basic Science Foundation. In 1998-2012 he was Head of the Laboratory of Functional Morphology of Hemoblastoses, Hematology Research Center (Moscow). Since 1995 he was appointed as Professor at the Dept. of Cell Biology and Histology (Moscow State University) teaching courses on Cell Biology, Cytoskeleton, Digital and Confocal Microscopy, Flow Cytometry. 



 Prof. Vorobyev collaborated worldwide: he was Research fellow at Beckman Laser Institute (University of California, Irvine, 1992), at University of Connecticut (2000-2001) was Co-PI on research grants with Prof. G. G. Borisy (University of Wisconsin, Madison and North-Western University, Chicago – 1997-2001), conducted research and taught a course on microscopy at University of Palermo (Italy, 1994-1995). Since 2005 he is collaborating with Dr. N. Barteneva (Boston Children’s Hospital-Harvard Medical School)


Research Interests
Prof. Ivan Vorobyev has many years of academic and research experience in the fields of biomedical research including: (1) centrosome fine structure and microtubule dynamics; (2) imaging and flow cytometry; (3) regulation of cell motility and anti-cancer treatments

SELECTED PUBLICATIONS (out of >160 articles in peer-reviewed journals)

Haridas V, Ranjbar S, Vorobjev IA, Goldfeld AE, Barteneva NS. 2016. Imaging flow cytometry analysis of intracellular pathogens. Methods. Sep 15. pii: S1046-2023(16)30311-5. doi: 10.1016/j.ymeth.2016.09.007. [Epub ahead of print] PMID: 27642004

Dashkova V, Malashenkov D, Poulton N, Vorobjev I, Barteneva NS. 2016. Imaging flow cytometry for phytoplankton analysis. Methods. pii: S1046-2023(16)30130-X. doi: 10.1016/j.ymeth.2016.05.007.

Vorobjev IA, Barteneva NS. 2016. Multi-parametric imaging of cell heterogeneity in apoptosis analysis. Methods. 2016 Jul 5. pii: S1046-2023(16)30205-5. doi: 10.1016/j.ymeth.2016.07.003.

Khvastunova AN, Kuznetsova SA,  Al-Radi LS, Vylegzhanina AV,  Zakirova AO, Fedyanina OS, Filatov AV, Vorobjev IA, Ataullakhanov F. 2015.  Anti-CD antibody microarray for human leukocyte morphology examination allows analyzing rare cell populations and suggesting preliminary diagnosis in leukemia. Scientific Reports 5: 12573. DOI: 10.1038/srep12573

Alieva I.B., Beresynskaya T.L., Borisy, G.G., Vorobjev I.A. 2015. Centrosome nucleates numerous ephemeral microtubules and only few of them participate in the radial array. Cell Biol Int. 39(11): 1203-1216. doi: 10.1002/cbin.10492. 

Vorobjev I.A., Barteneva N.S. 2015. Temporal heterogeneity metrics in apoptosis induced by anticancer drugs J Histochem. Cytochem. 63(7): 494-510. doi: 10.1369/0022155415583534.

Potashnikova D., Gladkikh A., and Vorobjev I. A. 2015. Selection of superior reference genes’ combination for quantitative real-time PCR in B-cell lymphomas. Annals of Clinical & Laboratory Science, 45 (1): 489-497. PMID: 25696013

Barteneva N. S., Ponomarev E., Tsytsykova A,, Armant M. and Vorobjev I.A. 2014. Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting. J Histochem. Cytochem. 62 (4): 265 — 276. doi: 10.1369/0022155413520404.

Mantel P.-Y., Hoang A.N., Goldowitz I., Potashnikova D., Hamza B., Vorobjev I., Ghhian I., Toner M., Irimia D., Ivanov A.R., Barteneva N., Marti M. 2013. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host & Microbe 13, 1–14. doi: 10.1016/j.chom.2013.04.009.

Barteneva N.S., Fasler-Kan E., Bernimoulin M., Stern J.NH, Ponomarev E. D., Duckett L. and Vorobjev I.A. 2013. Circulating microparticles: square the circle. BMC Cell Biology. 14: 23. 

Barteneva N. S., Ketman K., Fasler-Kan E., Potashnikova D., Vorobjev I. A. 2013. Cell sorting in cancer research – diminishing degree of cell heterogeneity. BBA Reviews on Cancer. 1836(1): 105-122. http://dx.doi.org/10.1016/j.bbcan.2013.02.004

Vorobjev I.A., Buchholz K., Prabhat P., Ketman K., Egan E., Marti M., Duraisingh M.T., Barteneva N.S. 2012. Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters. Malaria Journal. 11(1): 312.

Barteneva N.S., Fasler-Kan E., Vorobjev I.A. 2012. Imaging Flow Cytometry: Coping with Heterogeneity in Biological Systems. J Histochem. Cytochem. 2012. 60(10): 723-733.

Moskalev E.A., Luckert K., Vorobjev I.A., Mastitsky S.E., Gladkikh A.A., Stephan A., Schrenk M., Kaplanov K.D., Kalashnikova O.B., Pötz O., Joos T.O., Hoheisel J.D. 2012. Concurrent epigenetic silencing of Wnt/beta-catenin pathway inhibitor genes in B cell chronic lymphocytic leukaemia. BMC Cancer. 2012. 12(1): 213   (IF 3.01)

van der Vaart B, Manatschal C, Grigoriev I, Olieric V, Gouveia SM, Bjelic S, Demmers J, Vorobjev I, Hoogenraad CC, Steinmetz MO, Akhmanova A. 2011. SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase. J Cell Biol.; 193(6): 1083-1099.

Gladkikh A., Potashnikova D., Korneva E., Khudoleeva O., and Vorobjev I. 2010. Cyclin D1 expression in B-cell lymphomas. Experimental Hematology; 38: 1047–1057.

Nikitin EA, Malakho SG, Biderman BV, Baranova AV, Lorie YY, Shevelev AY, Peklo MM, Vlasik TN, Moskalev EA, Zingerman BV, Vorob’ev IA, Poltaraus AB, Sudarikov AB, Vorobjev AI. 2007. Expression level of lipoprotein lipase and dystrophin genes predict survival in B-cell chronic lymphocytic leukemia. Leuk Lymphoma. 48(5): 912-922.

Mimori-Kiyosue Y, Grigoriev I, Sasaki H, Matsui C, Akhmanova A, Tsukita S, Vorobjev I. 2006. Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment. Genes Cells. 11(8): 845-857.

Grigoriev I., Borisy G. and Vorobjev I. 2006. Regulation of microtubule dynamics in 3T3 fibroblasts by Rho family GTPases. Cell Motility and the Cytoskeleton, 63(1): 29–40.

Mimori-Kiyosue Yu., Grigoriev I., Lansbergen G., Sasaki H., Matsui C., Severin F., Galjart N., Grosveld F., Vorobjev I., Tsukita S., and Akhmanova A.. 2005. CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus end dynamics at the cell cortex. J. Cell Biology, 168(1): 141-153.

Sheval E.V., Churakova J.V., Dudnik O.A., Vorobjev I. A. 2004. Examination of the proliferative activity of tumor cells in human lymphoid neoplasms using a morphometric approach. — Cancer Cytopathology102 (3): 174-185.  

Alieva I.B. and Vorobjev I.A. 2004. Vertebrate primary cilia: a sensory part of centrosomal complex in tissue cells, but a «sleeping beauty» in cultured cells? Cell Biology International, 28: 139-150.

Maly I.V., and Vorobjev I.A. 2002. Centrosome-dependent anisotropic random walk of cytoplasmic granules. Cell Biology International. 26: 791-799

Komarova Y.A., Vorobjev I.A. and Borisy G.G. 2002. Life cycle of MTs: persistent growth in the cell interior, asymmetric transition frequencies and effects of the cell boundary. J. Cell Sci. 115 (17): 3527-3539

Vorobjev I., Malikov V. and Rodionov V.. 2001 Self-organization of a radial microtubule array by dynein-dependent nucleation of microtubules. Proc Natl Acad Sci U S A. 98(18): 10160-10165.

Shakulov V.R., Vorobjev I.A., Rubtsov Y.P., Chichkova N.V., and Vartapetian A.B. 2000. Interaction of yeast importin  with the NLS of prothymosin is insufficient to trigger nuclear uptake of cargos. Biopys. Biochem. Res. Comm. 274, 548-552

Vorobjev I. A., Rodionov V.I., Maly I.V. and Borisy G.G.1999. Contribution of plus and minus end pathways to microtubule turnover. J. Cell Science, 112 (14): 2277-2289.

Gorgidze L.A., Oshemkova S.A., and Vorobjev I.A. 1998. Blue light inhibits mitosis in tissue culture cells. Bioscience Reports, 18 (4): 215-224.

Vorobjev I. A., Svitkina T.M. and Borisy G.G. 1997. Cytoplasmic assembly of microtubules in cultured cells. J. Cell Science, 110(21): 2635-2645.

Rubtsov Yu.P., Zolotukhin A.S., Vorobjev I.A., Chichkova N.V., Pavlov N.A., Karger E.M., Evstafieva A.G., Felber B.K., Vartapetian A.B. 1997. Mutational analysis of human protymosin a reveals a bipartite localization signal. FEBS letters. 413: 135-141.

Uzbekov R.E., Votchal M.S., Vorobjev I.A. 1995. Role of the centrosome in mitosis: UV microirradiation study J. Photochem. Photobiol. (ser. B) 29(2-3): 163-170.

Vorobjev I.A., Liang H., Wright W.B. and Berns M.W. 1993. Optical trapping for chromosome manipulation: a wavelengh dependence of induced chromosome bridges. Biophysical Journal, 64 (2): 533-538.

Alieva I.B., Vaisberg E.A., Nadezhdina E.S. and Vorobjev I.A. 1992. Microtubule and intermediate filament patterns around the centrosome in interphase cells. In: «The centrosome.» Kalnins V.C. (ed.). Acad.Press 1992. p. 103-129.

Alieva I.B., Vorobjev I.A. 1991. Induction of multipolar mitoses in cultured cells: decay and restructuring of the mitotic apparatus; distribution of centrioles. Chromosoma, 100: 532-542.

Gudima G.O., Vorobjev I.A., Chentsov Yu.S. 1988. Centriolar location during blood cell spreading and motion in vitro: an ultrastructural analysis. J. Cell Science, 89: 225-241.

Vorobjev I.A., Nadezhdina E.S. 1987. The centrosome and its role in the organization of microtubules. Intern. Review of Cytology, 106: 227 — 293.

Vorobjev I.A., Zorov D.B. 1983. Diazepam inhibits cell respiration and induces fragmentation of mitochondrial reticulum. FEBS Letters, 163 (2): 311-314.

Vorobjev I.A., Chentsov Yu.S. 1983. The dynamics of reconstitution of microtubules around the cell center after cooling. Eur. Journal Cell Biology, 30 (2): 139-143.

Vorobjev I.A., Chentsov Yu.S. 1982. Centrioles in the cell cycle. I. Epithelial cells. J. Cell Biol., 98 (3): 938-949.

Vorobjev I.A., Chentsov Yu.S. 1980. The ultrastructure of centriole in mammalian tissue culture cells. Cell Biology Intern. Reports, 4 (11): 1037-1044.