The study of gut and oral microbiome in patients with Alzheimer’s disease

«The study of gut and oral microbiome in patients with Alzheimer's disease»

IRN  AP09259730-KC-21 

 

Introduction:

 One of the important factors influencing human health and attracting increasing attention of the scientists during the last two decades is the human microbiome. Human microbiome is the communities of the microorganisms residing on or within human bodies. These communities have a tremendous impact on the human physiology in healthy individuals and during an illness. Recent studies have revealed significant changes of gut and oral microbiome in AD patients compared to healthy seniors. However, qualitative changes in the oral and intestinal microbiome in AD patients from different countries are not similar. Since the Asian population is heterogeneous, the microbiome biomarkers in the Kazakhstan population might be different from previously published ones, including from other Asian populations and, this is important for development of prognostics and preventive measures. Therefore, more research is needed in order to reveal the relationships existing between human microbiome and the risks of AD.

The project goal   

The project goal   is to study the diversity and composition of gut and oral microbiome isolated from local patients diagnosed with AD in comparison with healthy seniors in order to advance the understanding of the pathology and search for new diagnostic biomarkers and prophylactics strategies based on the analysis of microbiome.

Expected results:

  • Patients with a diagnosis of AD and healthy people, age and gender matched to the patients will be recruited;
  • The bacterial diversity and composition of gut microbiome of patients with AD will be characterized;
  • The bacterial diversity and composition of oral microbiome of patients with AD will be characterized;
  • The associations between the human gut and oral microbiome and the risk of AD will be identified;
  • Based on the results of the implementation of the projects for the entire period of the project, the following minimum results will be obtained:

- at least 3 (three) articles and (or) reviews in peer-reviewed scientific journals indexed in the Science Citation Index Expanded of the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 35 (thirty-five);

- either at least 2 (two) articles and (or) reviews in peer-reviewed scientific journals indexed in the Science Citation Index Expanded of the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 35 (thirty five), and not less than 1 (one) patent included in the Derwent Innovations Index (Web of Science, Clarivate Analytics);

- as well as at least 1 (one) article or review in a peer-reviewed foreign or domestic publication recommended by ССSES;

- either at least 2 (two) articles and (or) reviews in peer-reviewed scientific journals included in the 1 (first) and (or) 2 (second) quartiles in the Web of Science database and (or) having the CiteScore percentile in the Scopus database not less than 65 (sixty five);

- either at least 1 (one) article or review in a peer-reviewed scientific publication included in the 1 (first) quartile in the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 80 (eighty)

- supposedly, manuscripts will be published in the following journals: Frontiers in Cellular and Infection Microbiology (Q1, percentile 80%) and/or Journal of Alzheimer's Disease (Q1, percentile 91%)

- in addition, the results obtained in this project will be presented at the prestigious conferences in the field of Alzheimer’s and microbiome research.

 

Current results:

The research algorithm was designed and clinical questionnaires were developed to collect medical and demographic data, data for the analysis of nutrition, lifestyle and risk factors. Recruiting participants diagnosed with Alzheimer's disease to the study group was initiated. At the moment, 77 people have been recruited into the group of participants with Alzheimer's disease. The recruitment of a control group of conditionally healthy elderly people who do not suffer from cognitive impairments will be carried out in the next year of the project. Analysis of demographic and clinical data showed the average age of recruited participants to be 67 years old in the sample from Nur-Sultan city and 65 years old in Almaty city. More than half of the study participants are women and Kazakhs. About 53% of study participants have high blood pressure, 30% have coronary heart disease, and about 20% have a history of diabetes mellitus. Cognitive performance in study participants was assessed using the mental status short scale, the clock drawing test, and the neurological status assessment. The average MMSE score was 14 points. Also, a survey was conducted on socio-demographic data, lifestyle and nutrition; samples of biological material were collected. Extraction of bacterial DNA was performed from the stool of the study participants, quantitative and qualitative analysis of the isolated DNA samples was carried out. All selected samples were added to the DNA bank. Sample preparation was carried out using the Nextflex 16s V1-V3 Amplicon-seq KIT and sequencing at the 16s locus. The preliminary results of the analysis of taxonomic classification revealed a high individual variability in the relative number of microbes at the genus and phylum levels.

Research group:

Full name

Identifier

Links

Askarova Sholpan, PhD, professor

Scopus Author ID:  36443225300

 

ORCID: https://orcid.org/0000-0001-6161-1671

 

Web of Science Researcher ID: P-1091-2017

 

https://www.scopus.com/authid/detail.uri?authorId=36443225300

https://orcid.org/0000-0001-6161-1671

https://www.webofscience.com/wos/author/record/2812301,31280432

Kaiyrlykyzy Aiym, MD, MPH

Scopus Author ID:  56566707600

 

ORCID: 56566707600

Web of Science ResearcherID: F-5469-2018

 

https://www.scopus.com/authid/detail.uri?authorId=56566707600

https://orcid.org/my-orcid?orcid=0000-0002-4911-1671

http://www.researcherid.com/rid/F-5469-2018

Olzhayev Farkhad, MD

Scopus Author ID:  56605070400

 

ORCID: 0000- 0000-0002-1906-6654

 

Web of Science ResearcherID: P-2121-2015

 

https://www.scopus.com/authid/detail.uri?authorId=56605070400

https://orcid.org/0000-0002-1906-6654

https://www.webofscience.com/wos/author/record/8052262

Andrey Tsoy, PhD

Scopus Author ID:56362596800

 

Web of Science Researcher ID:

K-3983-2014

https://www.scopus.com/authid/detail.uri?authorId=56362596800

https://publons.com/researcher/2494656/andrey-tsoy/

 

Publications in 2021:

Askarova, S. (2021). The Links Between the Gut Microbiome, Aging, Modern Lifestyle and Alzheimer’s Disease. Hill-Yardin. E.L. (Ed). Interactions of the Nervous System with Bacteria (pp 115-126). Lausanne: Frontires Media SA. Doi:10.2289/978-2-88966-903-5

S Askarova, A Kaiyrlykyzy, S Kozhakhmetov, D Babenko, F Olzhayev. Gut microbiome alterations in Alzheimer’s patients from Kazakhstan. 2021 Alzheimer's Association International Conference Proceedings. https://alz.confex.com/alz/2021/meetingapp.cgi/Paper/52741