Immunoregulatory role of gut microbiome and mycobiome in aging

Project name: Immunoregulatory role of gut microbiome and mycobiome in aging

IRN АР 09058099

 

Relevance. In healthy aging, centenarians are a successful model, exhibiting relatively low rates of various diseases and improved long-term health compared to older people. In the aging process, variation in the gut microbiome plays a key role, which is facilitated by the diet, genetics and lifestyle of older people. In this regard, the study of the gut microbiome / mycobiome of long-lived people may lead to an understanding of how, with increasing longevity, the gut microbiota adapts to changes and contributes to the maintenance of metabolic and immunological homeostasis of the body, as well as supports quality aging and increases the survival of the body.

The goal of the project is to investigate the local intestinal immune status and its aging role in people over 95 years old living in Kazakhstan.

Expected and achieved results

The results of the project contribute to an increase in average life expectancy and qualitative aging, which is an actual characteristic of the well-being of the population. As part of the project, a comprehensive clinical and laboratory examination of people over 95 years old and people of different age categories will be carried out, with the taking and interpretation of the relevant tests (blood, rectal swabs); an assessment of the common and local immune status of study participants over 95 years old and a control group of study participants of different age categories will be carried out; the compositional microbial composition of the intestines of persons over 95 years old and the control group of different age categories will be studied; A genetic biomarker of intestinal senescence (protein p16) will be determined in study participants over 95 and in other age groups; correlation analysis of indicators of common and local immune status and microbiome / mycobiome will be carried out.

During the reporting period, work was completed on recruiting people over 95 years old, who were included in the study according to the inclusion and exclusion criteria. According to the task and the design of the study, the sample for the research group was formed using the register of the attached population by accessibility from the clinics of the cities of Nur-Sultan and Karaganda. With the participation of qualified medical workers, a comprehensive survey of participants, assessment of the general state of health, collection of anamnesis of life, anamnesis of the disease was conducted at the respondents' home The most common somatic, mental and social geriatric syndromes were studied according to international scales and questionnaires (IADL, Bartel, FRAIL, PASE scales and SARC-F questionnaire) used to assess the geriatric status of respondents. Blood and urine sampling was carried out at the respondents' homes with the departure of the medical staff of the Olymp CDL for the study of clinical and laboratory parameters (analysis of general blood analysis, general urine analysis and biochemical parameters). The medical staff collected feces and a colon smear for further research. A questionnaire was conducted related to the image and nature of FFQ nutrition. The work on recruiting individuals to the control group of different age categories and comprehensive clinical and laboratory examination has been completed. Biomaterial was collected (blood, fecal and rectal swabs). Based on the data on average life expectancy, the control group included respondents aged 70-89 years of age with voluntary consent. The sample was formed from the rural districts of Torgai (Kostanay region), the village of Zhansary (Karaganda region), the village of Rodina (Akmola region) and the cities of Temirtau, Karaganda (Karaganda region) and Nur-Sultan. According to the questionnaire, work was carried out to collect primary information, a comprehensive examination, an assessment of general health, physical activity, cognitive function and nutrition was carried out. Biological material was collected from the study participants by qualified medical personnel in disposable plastic vacuum tubes. Clinical and laboratory examination of respondents included studies of biochemical parameters of blood. Fecal were collected in disposable containers to study the microbiome and microbial composition of the intestine.

 

 

Research group:

Full name

Participation in the project

Scientist/academic degree

ORCID

Scopus Authors ID

Research ID

Chulenbayeva Laura

PI

PhD

https://orcid.org/

0000-0002-8691-9485

57205369003

https://publons.com/researcher/ABE-9582-2021/

Kozhakhmetov Samat

Project executor

PhD, Associate Professor

https://orcid.org/0000-0001-9668-0327

57223210171

https://publons.com/researcher/N-4128-2017/

Sergazy Shynggys.

Project executor

PhD.

https://orcid.org/0000-0002-6030-620X

57053283300

 

https://publons.com/researcher/AAR-6438-2020/

Nurgozhina Ayaulym

Project executor

Master in biology

https://orcid.org/ 0000-0001-7042-4763

57207769556

 

 

Nurgazyev Madiyar

Project executor

Master in biology

https://orcid.org/0000-0003-2397-4978

57216501021

https://publons.com/researcher/AAV-9220-2020/

Ganzhula Yuliya

Project executor

Master in medcine

https://orcid.org/0000-0002-6030-620

57053283300

 

https://publons.com/researcher/AAR-6438-2020/

Zhetkenev Sanzhar

Project executor

Master in biology

https://orcid.org/0000-0002-0627-6118

 

 

Mukhanbetzhanov Nurislam

Project executor

Master in biology

https://orcid.org/0000-0002-0627-6118

57221370132

https://publons.com/researcher/ABB-2928-2021

Akhmetova Yekaterina

Project executor

 

https://orcid.org/0000-0001-5923-6905

 

https://publons.com/researcher/ABF-2017-2021